» Antibodies » anti-CD14 Molecule (CD14) antibody (APC)

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CD14 Molecule (CD14) antibody (APC)

Antigen	CD14 Molecule (CD14)
Synonyms	CD14
Clonality	Monoclonal (MEM-18)
Host	Alternatives Mouse Mouse (240), Rabbit (36), Goat (8), Rat (2), Chicken (1)
Reactivity	Alternatives Human Human (265), Pig (Porcine) (34), Mouse (Murine) (30), Cow (Bovine) (28), Dog (Canine) (26), Sheep (Ovine) (25), Rabbit (24), Monkey (22), Primates (19), Rat (Rattus) (18), Goat (9), Cat (Feline) (8), Primate (8), Chicken (1), Llama (1)
Conjugate	Alternatives APC FITC (35), PE (24), Biotin (21), APC (15), RPE (8), Cy5.5 (5), Alexa Fluor 488 (3), Pacific Blue (3), Alexa Fluor 700 (2), Cy5 (2), Cy7,PE (2), PE,Cy5 (2), PE,Cy7 (2), PE,DY590 (2), PE,DY647 (2), SPRD (2), APC,Cy7 (1), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), CF (1), Cy3 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5.5 (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PerCP (1), RBITC (1), RPE,Cy5 (1), RPE,Cy5.5 (1)
Application	Alternatives Flow Cytometry (FACS) Flow Cytometry (FACS) (217), Western Blotting (WB) (55), ELISA (37), Immunoprecipitation (IP) (32), Functional Studies (Func) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (24), Immunohistochemistry (Frozen Sections) (IHC (fro)) (22), Immunofluorescence (IF) (20), Enzyme Immunoassay (EIA) (19), Immunohistochemistry (IHC) (16), ELISA (Detection) (5), Blocking Antibody (Inhibition) (3), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Radioimmunoassay (RIA) (1)

Catalog no.	ABIN238454
Quantity	100 Tests
Price	330.00 $ Plus shipping costs $35.00
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Availability	Ships within 7 to 10 Business Days

Additional Information
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Alternative name	CD14
Swiss-Prot	P08571
Immunogen	A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria
Cross-Reactivity	Human, Monkey
Isotype	IgG1
Clone	MEM-18
Description	CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor, it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipo-polysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipo- polysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes. Alternate Names: LPS-receptor.
Specificity	The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes, also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.

Application Details
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Application Notes	Suitable for Flow Cytometry analysis of human blood cells using 10 m l reagent / 100 m l of whole blood or 10e6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Buffer	PBS, pH 7.4 with 15 mM sodium azide as preservative and 0.2% (w/v) high- grade BSA (protease free) as a stabilizer.
Storage	Store the antibody in the dark at 2-8°C. Do Not freeze! Avoid prolonged exposure to light. Shelf Life: One year from despatch.
Research Area	Stem Cells, Innate Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Immunology, Inflammation
Restrictions	For Research Use only

Alternatives
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Alternatives for antigen "CD14 Molecule (CD14)", type "Antibodies"

Hosts	Mouse (240), Rabbit (36), Goat (8), Rat (2), Chicken (1)
Reactivities	Human (265), Pig (Porcine) (34), Mouse (Murine) (30), Cow (Bovine) (28), Dog (Canine) (26), Sheep (Ovine) (25), Rabbit (24), Monkey (22), Primates (19), Rat (Rattus) (18), Goat (9), Cat (Feline) (8), Primate (8), Chicken (1), Llama (1)
Applications	Flow Cytometry (FACS) (217), Western Blotting (WB) (55), ELISA (37), Immunoprecipitation (IP) (32), Functional Studies (Func) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (24), Immunohistochemistry (Frozen Sections) (IHC (fro)) (22), Immunofluorescence (IF) (20), Enzyme Immunoassay (EIA) (19), Immunohistochemistry (IHC) (16), ELISA (Detection) (5), Blocking Antibody (Inhibition) (3), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Radioimmunoassay (RIA) (1)
Conjugates	FITC (35), PE (24), Biotin (21), APC (15), RPE (8), Cy5.5 (5), Alexa Fluor 488 (3), Pacific Blue (3), Alexa Fluor 700 (2), Cy5 (2), Cy7,PE (2), PE,Cy5 (2), PE,Cy7 (2), PE,DY590 (2), PE,DY647 (2), SPRD (2), APC,Cy7 (1), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), CF (1), Cy3 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5.5 (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PerCP (1), RBITC (1), RPE,Cy5 (1), RPE,Cy5.5 (1)
Epitopes	C-Term (2), N-Term (2)