S100 Calcium Binding Protein A9 (S100A9) antibody

Antigen: S100 Calcium Binding Protein A9 (S100A9)

Synonyms: MIF, NIF, P14, CAGB, CFAG, CGLB, L1AG, LIAG, MRP14, 60B8AG, MAC387, Mrp14, S100A9, MGC137042

Clonality: Monoclonal (S32-2)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN238498

Additional Information

Characteristics: Synonyms: S100-A9, CAGB, MRP-14, S100 calcium-binding protein A9, Migration inhibitoryfactor-related protein 14, Leukocyte L1 complex heavy chain, Calprotectin L1H subunit

Alternative name: S100A9 / Calgranulin-B / MRP14

Swiss-Prot: P06702

Immunogen: Cultured human monocytes.

Cross-Reactivity: Human

Isotype: IgG1  (Matching secondary antibodies)

Clone: S32-2

Description: S100A9 is a member of the S100 family of proteins. S100A9, together with S100A8 forms aheterodimeric protein complex, Calprotectin, which is a major calcium- and zinc-bindingprotein in the cytosol of neutrophils, monocytes, and keratinocytes. Complexes of S100A8and S100A9 are the physiologically relevant forms of these proteins. S100A9 may function in the inhibition of casein kinase and altered expression of thisprotein is associated with the disease cystic fibrosis. Its expression and potential cytokine-like function in inflammation and in cancer suggestthat S100A8/A9 may play a key role in inflammation-associated cancer.

Specificity: Monoclonal antibody S32. 2 identifies the Ca2+ -binding 14kD subunit of the inflammatoryL-1 protein complex, also called S100A9 or Calgranulin B. The antigen is MRP14, theepitope is suspected in the carboxyterminal portion of the peptide. The antibody is useful for the characterization of circulating granulocytes or inflammatoryinfiltrates of the myelo-monocytic lineage which express MRP14 differently depending on

Application Details

Application Notes: ELISA. Immunohistochemistry on Frozen Sections: 0.5 µg/ml (1/400). Immunohistochemistry on Paraffin Sections: 1 µg/ml (1/200), pre-treatment for antigenretrieval not required. Has been described to work in FACS and Dot blots. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.2 mg/ml (after reconstitution)

Purification: Purified

Buffer: Stock soöution conatins PBS, pH 7.2 with 10 mg/ml BSA, 0.05% Luviskol asstabilizer and 0.01% Thimerosal as a preservative.Reconstitution: Restore with 0.5 ml distilled water.

Storage: Store the original vial at 2-8°C. Store stock solution or aliquots thereof at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only