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Antibodies
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anti-HLA Class I ABC antibody (Biotin)
HLA Class I ABC antibody (Biotin)
| Antigen |
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| Clonality |
Monoclonal (W6-32) |
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Host
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Reactivity
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Conjugate
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Application
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| Catalog no. |
ABIN238525 |
| Quantity |
0.1 mg (1.0 mg/ml) (Variants) |
| Price |
380.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: HLA-ABC, HLA class I histocompatibility antigen, MHC class I antigen
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Immunogen
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Membrane of human tonsil cellsRemarks: Gene Locus: 6p21. 3
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Cross-Reactivity
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Cow (Bovine), Cat (Feline), Human
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Isotype
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IgG2a
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Clone
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W6-32
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Description
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HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteinsexpressed on nucleated cells and noncovalently associated with an invariant beta-2microglobulin. They carry foreign determinants important for immune recognition bycytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class Iantigens are represented by HLA-A, HLA-B and HLA-C molecules. MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types.
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Specificity
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The antibody W6/32 recognizes virtually all nucleated human cells. It is a valuable reagentfor analysing variations in HLA class I expression in different disease states e. g. liverdisease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody W6/32 is also suitable as a positive control for HLA tissue typing andcrossmatching. Species: Human, Non-Human Primates, Bovine, Feline (Cat). Does not work in Rabbit. Others not tested.
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Application Notes
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Flow Cytometry: (1/300 starting dilution). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Buffer
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Phosphate buffered saline (PBS) with 15 mM Sodium Azide as preservative,approx. pH 7.4Label: Biotin-LC-NHS, free of unconjugated Biotin
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Research Area
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Major Histocompatibility Complex (MHC)
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Restrictions
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For Research Use only
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Alternatives for antigen "HLA Class I ABC", type "Antibodies"
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Hosts
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Mouse (22)
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Reactivities
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Human (18), Cow (Bovine) (14), Cat (Feline) (9), Primate (9), Monkey (7), Chimpanzee (1)
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Applications
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Flow Cytometry (FACS) (20), Immunoprecipitation (IP) (7), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Enzyme Immunoassay (EIA) (4), Western Blotting (WB) (3), Functional Studies (Func) (1)
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Conjugates
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FITC (5), PE (4), Biotin (2), HRP (1)
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Alternatives 
