Casein Kinase 1, epsilon (CSNK1E) antibody

Antigen: Casein Kinase 1, epsilon (CSNK1E)

Synonyms: CKIe, hckie, CKIepsilon, ck1-epsilon, CSNK1E, Csnk1e, dco, MGC143296, fj38c01, MGC77310, zgc:77310, wu:fj38c01, wu:fj62a04, HCKIE, MGC10398, tau, AI426939, AI551861, AW457082, CK1epsilon, KC1epsilon

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238540

Additional Information

Alternative name: CSNK1E

Gene ID: 1454, 27373 (mouse), 58822 (rat)

Immunogen: C-PASQTSVPFDHLGK

Format: Affinity-purified

Description: All names and symbols: CSNK1E, casein kinase 1, epsilon, RP1-5O6.1, HCKIE, MGC10398 , CKI-epsilon, OTTHUMP00000063258, OTTHUMP00000063263, casein kinase 1 epsilon, casein kinase I epsilon. Official Symbol: CSNK1E. RefSeq number(s): NP_001885.1, NP_689407.1

Specificity: Both reported variants represent identical protein (NP_001885.1 and NP_689407.1). Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 45kDa band observed in lysates of cell line HEK293 (calculated MW of 47.3kDa according to NP_001885.1 and NP_689407.1). In transfected HEK293 transiently expressing CSNK1E a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN238540 (1µg/ml) staining of HEK293 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing CSNK1E (RC202436) and probed with ABIN238540 (mock transfection in first lane), tested by Origene.

Publications

Publications: Haemmerlein, Weiske, Huber: "A second protein kinase CK1-mediated step negatively regulates Wnt signalling by disrupting the lymphocyte enhancer factor-1/beta-catenin complex." in: Cellular and molecular life sciences : CMLS, Vol. 62, Issue 5, pp. 606-18, 2005 (PubMed).