KCNJ11 antibody (Internal Region)

Catalog No. ABIN238541

The Goat Polyclonal anti-KCNJ11 antibody has been validated for WB, ELISA and IHC. It is suitable to detect KCNJ11 in samples from Human.

Quick Overview for KCNJ11 antibody (Internal Region) (ABIN238541)

Target: KCNJ11 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 11 (KCNJ11))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This KCNJ11 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-KCNJ11 Antibody

Binding Specificity: Internal Region

Purpose: KCNJ11 / KATP /

Sequence: AEDPAKPRYR ARQ

Specificity: This antibody is expected to recognise isoform 1 (NP_000516.3) only.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-AEDPAKPRYRARQ, from the internal region (near the N Terminus) of the protein sequence according to NP_000516.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Pancreas shows variable staining across the islet of Langerhans. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 45-48 kDa band observed in Human Skeletal Muscle lysates (calculated MW of 43.5 kDa according to NP_000516.3). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for KCNJ11

Target: KCNJ11 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 11 (KCNJ11))

Alternative Name: KCNJ11

Background: KCNJ11, IKATP, potassium inwardly-rectifying channel, subfamily J, member 11, BIR, HHF2, KIR6.2, MGC133230, PHHI, TNDM3, ATP-sensitive inward rectifier potassium channel 11, beta-cell inward rectifier subunit, inwardly rectifying potassium channel KIR6.2,

Gene ID: 3767

NCBI Accession: NP_000516

Pathways: Negative Regulation of Hormone Secretion