Neuron Derived Neurotrophic Factor (NENF) antibody

Antigen: Neuron Derived Neurotrophic Factor (NENF)

Synonyms: CIR2, SPUF, SCIRP10, NEUDESIN, Spuf, 1110060M21Rik, sp2, MGC112953, MGC152703, wu:fa11a04, wu:fb73f07, zgc:112953, zgc:152703, cir2, spuf, scirp10, MGC108468, NENF, MGC139431

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238554

Additional Information

Alternative name: Neudesin / NENF

Gene ID: 29937, 66208 (mouse), 289380 (rat)

Immunogen: C-NEDGSPNLDFKP

Format: Affinity-purified

Description: All names and symbols: NENF, neuron derived neurotrophic factor, CIR2, SCIRP10, SPUF, SCIRP10-related protein, Spinal cord injury related protein 10 , cell growth-inhibiting protein 47 neudesin secreted protein of unknown function, Neudesin,. Official Symbol: NENF. RefSeq number(s): NP_037481.1

Specificity: Human, Mouse, Rat, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx 45kDa band in Human Brain (Cerebellum) lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 18.6kDa according to NP_037481.1 The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Kimura, Konishi, Miyake et al.: "Neudesin, a secreted factor, promotes neural cell proliferation and neuronal differentiation in mouse neural precursor cells." in: Journal of neuroscience research, Vol. 83, Issue 8, pp. 1415-24, 2006 (PubMed).