beta-1,3-N-Acetylglucosaminyltransferase 2 (B3GNT2) antibody

Antigen: beta-1,3-N-Acetylglucosaminyltransferase 2 (B3GNT2)

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238559

Additional Information

Alternative name: B3GNT2

Gene ID: 10678, 53625 (mouse)

Immunogen: C-EKHKGFRTFDIE

Format: Affinity-purified

Description: All names and symbols: B3GNT2, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 , B3GN-T1, B3GN-T2, B3GNT, B3GNT-2, B3GNT1, BETA3GNT, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1, beta-1,3-N-acetylglucosaminyltransferase bGnT-1, beta-1,3-N-a. Official Symbol: B3GNT2 . RefSeq number(s): NP_006568.2

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 32,000. Western Blot: Approx 45kDa band observed in Human Pancreas lysates (calculated MW of 46.0kDa according to NP_006568.2). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN238559 (0.1µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Hayashi, Nakamori, Okami et al.: "Association between expression levels of CA 19-9 and N-acetylglucosamine-beta;1,3-galactosyltransferase 5 gene in human pancreatic cancer tissue." in: Pathobiology : journal of immunopathology, molecular and cellular biology, Vol. 71, Issue 1, pp. 26-34, 2003 (PubMed).