MEPCE antibody (Internal Region)

Catalog No. ABIN238571

The Goat Polyclonal anti-MEPCE antibody is suitable to detect MEPCE in samples from Human and Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for MEPCE antibody (Internal Region) (ABIN238571)

Target: MEPCE (Methylphosphate Capping Enzyme (MEPCE))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This MEPCE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-MEPCE Antibody

Binding Specificity: Internal Region

Purpose: MEPCE

Sequence: KQQRKFQYGN YCK

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: KQQRKFQYGNYCK

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Testis shows staining of the spermatids. Recommended concentration: 5-10 μg/mL.

Western Blot: Approx. 75 kDa band observed in Rat Testis lysates (calculated MW of 74.4 kDa according to Human NP_062552.2 and 75.3 kDa according to Rat XP_222048.4). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. Preliminary testing wa

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MEPCE

Target: MEPCE (Methylphosphate Capping Enzyme (MEPCE))

Alternative Name: MEPCE

Background: MEPCE, methylphosphate capping enzyme, BCDIN3, FLJ20257, bin3, bicoid-interacting 3, bin3, bicoid-interacting 3, homolog

Gene ID: 56257

NCBI Accession: NP_062552

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection