Methylphosphate Capping Enzyme (MEPCE) antibody

Antigen: Methylphosphate Capping Enzyme (MEPCE)

Synonyms: BCDIN3, FLJ20257, Bcdin3, D5Wsu46e, MGC28888, MGC30590, MEPCE, fc59f06, wu:fc59f06, si:ch211-283h6.1, DKFZp459J0215

Clonality: Polyclonal

Host: Goat

Reactivity: Rat (Rattus), Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238571

Additional Information

Alternative name: MEPCE

Gene ID: 56257

Immunogen: KQQRKFQYGNYCK

Format: Affinity-purified

Description: All names and symbols: MEPCE, methylphosphate capping enzyme, BCDIN3, FLJ20257, bin3, bicoid-interacting 3, bin3, bicoid-interacting 3, homolog. Official Symbol: MEPCE . RefSeq number(s): NP_062552.2

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: Approx. 75kDa band observed in Rat Testis lysates (calculated MW of 74.4kDa according to Human NP_062552.2 and 75.3kDa according to Rat XP_222048.4). In transfected HEK293 transiently expressing Human MEPCE a band of approx. 75kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, DNA/RNA

Restrictions: For Research Use only

Images

ABIN238571 (1µg/ml) staining of Rat Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing MEPCE (RC200948) and probed with ABIN238571 (mock transfection in first lane), tested by Origene.

Publications

Publications: Jeronimo, Forget, Bouchard et al.: "Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme." in: Molecular cell, Vol. 27, Issue 2, pp. 262-74, 2007 (PubMed).