Polypyrimidine Tract Binding Protein 2 (PTBP2) antibody

Antigen: Polypyrimidine Tract Binding Protein 2 (PTBP2)

Synonyms: PTB, nPTB, PTBLP, brPTB, nPTB5, nPTB6, nPTB7, nPTB8, FLJ34897, Ptb2, MGC95244, PTBP2, MGC139378, ptbp2, wu:fa08f05, wu:fc05d10, si:ch211-160l17.2

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238583

Additional Information

Alternative name: PTBP2

Gene ID: 58155, 56195 (mouse), 310820 (rat)

Immunogen: C-SKKFKGEDKMD

Format: Affinity-purified

Description: All names and symbols: PTBP2, polypyrimidine tract binding protein 2, FLJ34897, MIBP, PTB, PTBLP, brPTB, nPTB, nPTB5, nPTB6, nPTB7, nPTB8, PTB-like, neural polypyrimidine tract binding protein, splicing regulator. Official Symbol: PTBP2 . RefSeq number(s): NP_067013.1

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Preliminary experiments gave an approx 35kDa band in lysates of cell line NIH3T3 after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.5kDa according to NP_067013.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Chromatin Binding Proteins, DNA/RNA

Restrictions: For Research Use only

Publications

Publications: Boutz, Stoilov, Li et al.: "A post-transcriptional regulatory switch in polypyrimidine tract-binding proteins reprograms alternative splicing in developing neurons." in: Genes & development, Vol. 21, Issue 13, pp. 1636-52, 2007 (PubMed).