erythrocyte Membrane Protein Band 4.1-Like 2 (EPB41L2) (AA 593-604) antibody

Antigen: erythrocyte Membrane Protein Band 4.1-Like 2 (EPB41L2)

Synonyms: 4.1B, DAL1, DAL-1, FLJ37633, KIAA0987, 4.1-G, DKFZp781D1972, DKFZp781H1755, EPB41L2, DKFZp459F2232

Epitope: AA 593-604

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238594

Additional Information

Alternative name: EPB41L2 / 4.1G (aa 593 to 604)

Gene ID: 2037

Immunogen: C-RREVRSPTKAPH

Format: Affinity-purified

Description: All names and symbols: EPB41L2, 4.1-G, erythrocyte membrane protein band 4.1-like 2, RP3-324N14.1, DKFZp781D1972, DKFZp781H1755, OTTHUMP00000040262, erythrocyte membrane protein band 4.1 like-protein 2. Official Symbol: EPB41L2 . RefSeq number(s): NP_001422.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 110kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 113kDa according to NP_001422.1). Recommended concentration: 0.03-0.1µg/ml. An additional double band of unknown identity was also consistently observed at 38-40kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN238594 (0.1µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Ralston, Hird, Zhang et al.: "The LFA-1-associated molecule PTA-1 (CD226) on T cells forms a dynamic molecular complex with protein 4.1G and human discs large." in: The Journal of biological chemistry, Vol. 279, Issue 32, pp. 33816-28, 2004 (PubMed).