erythrocyte Membrane Protein Band 4.1 Like 4B (EPB41L4B) antibody

Antigen: erythrocyte Membrane Protein Band 4.1 Like 4B (EPB41L4B)

Synonyms: CG1, EHM2, FLJ21596, DKFZp761N1814, Epb41l4b

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238595

Additional Information

Alternative name: EPB41L4B / EHM2

Gene ID: 54566

Immunogen: C-HNYQPQYHPNIHPSQ

Format: Affinity-purified

Description: All names and symbols: EPB41L4B, erythrocyte membrane protein band 4.1 like 4B, CG1, DKFZp761N1814, EHM2, FLJ21596, EHM2, FERM-containing protein. Official Symbol: EPB41L4B . RefSeq number(s): NP_060894.2, NP_061987.3

Specificity: This antibody is expected to recognise both reported isoforms (NP_060894.2, NP_061987.3). Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 48kDa band in Human Brain (Hippocampus, Cerebellar Cortex, Cerebellum) and Human Bone Marrow lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 58.6kDa according to NP_060894.2. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Diakowski, Grzybek, Sikorski: "Protein 4.1, a component of the erythrocyte membrane skeleton and its related homologue proteins forming the protein 4.1/FERM superfamily." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 44, Issue 4, pp. 231-48, 2007 (PubMed).