tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide (YWHAQ) antibody

Antigen: tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide (YWHAQ)

Synonyms: HS1, GW128, KCIP-1, 1C5, 14-3-3, R74690, AA409740, AU021156, MGC118161, 2700028P07Rik, 14-3-3t, MGC53015, 1c5, hs1, MGC69491, YWHAQ, MGC127486, DKFZp459M2030

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238596

Additional Information

Alternative name: 14-3-3 tau / YWHAQ

Gene ID: 10971

Immunogen: C-DDRKQTIDNSQ

Format: Affinity-purified

Description: All names and symbols: YWHAQ, 14-3-3 protein tau, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide, 14-3-3, 1C5, HS1, 14-3-3 protein T-cell, 14-3-3 protein theta, protein tau, tyrosine 3/tryptophan 5 -monooxygenase activation protein, theta polypeptide. Official Symbol: YWHAQ . RefSeq number(s): NP_006817.1

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Approx 28kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 27.8kDa according to NP_006817.1). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN238596 (0.03µg/ml) staining of Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Howlett, Sakamoto, Garnham et al.: "Resistance exercise and insulin regulate AS160 and interaction with 14-3-3 in human skeletal muscle." in: Diabetes, Vol. 56, Issue 6, pp. 1608-14, 2007 (PubMed).