Membrane-Associated Ring Finger (C3HC4) 2 (MARCH2) antibody

Antigen: Membrane-Associated Ring Finger (C3HC4) 2 (MARCH2)

Synonyms: RNF172, HSPC240, MARCH-II, march2, rgd1306395, marh2, RGD1306395

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238602

Additional Information

Alternative name: MARCH2 / MARCH-II

Gene ID: 51257

Immunogen: C-KIREADSPEGPQH

Format: Affinity-purified

Description: All names and symbols: MARCH2, membrane-associated ring finger (C3HC4) 2, HSPC240, MARCH-II, RNF172, membrane-associated RING-CH protein II. Official Symbol: 01.03.02. RefSeq number(s): NP_001005415.1, NP_001005416.1, NP_057580.3

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments in Human Heart, Skeletal Muscle, Lung and Spleen lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Nakamura, Fukuda, Kato et al.: "MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking." in: Molecular biology of the cell, Vol. 16, Issue 4, pp. 1696-710, 2005 (PubMed).