Naked Cuticle Homolog 2 (Drosophila) (NKD2) antibody

Antigen: Naked Cuticle Homolog 2 (Drosophila) (NKD2)

Synonyms: AW212591, 2210403L10Rik, Naked2, nkd2b

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238609

Additional Information

Alternative name: NKD2

Gene ID: 85409, 72293 (mouse), 308068 (rat)

Immunogen: C-HKRYRQKGREGHS

Format: Affinity-purified

Description: All names and symbols: NKD2, naked cuticle homolog 2 (Drosophila), Dvl-binding protein NKD2, naked cuticle homolog 2, naked cuticle-2. Official Symbol: NKD2. RefSeq number(s): NP_149111.1

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave bands at approx 80kDa, 38kDa and 26kDa in Mouse Brain lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 51.5kDa according to Mouse NP_082462.2 and of 50.1kDa according to Human NP_149111.1. All the detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Stem Cells, Wnt

Restrictions: For Research Use only

Publications

Publications: Zhang, Cagatay, Amanai et al.: "Viable mice with compound mutations in the Wnt/Dvl pathway antagonists nkd1 and nkd2." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4454-64, 2007 (PubMed).