Hydroxyacyl-CoA Dehydrogenase (HADH) antibody

Antigen: Hydroxyacyl-CoA Dehydrogenase (HADH)

Synonyms: HAD, HHF4, HADH1, SCHAD, HADHSC, M/SCHAD, MGC8392, Hadhsc, AA409008, AU019341, AW742602, hadhsc, MGC69125, zgc:86777, wu:fb66a11, HADH

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238619

Additional Information

Alternative name: HADH / HADHSC

Gene ID: 3033, 15107 (mouse)

Immunogen: C-YERGDASKEDID

Format: Affinity-purified

Description: All names and symbols: HADH, HADHSC, hydroxyacyl-Coenzyme A dehydrogenase, HAD, HADH1, HHF4, M/SCHAD, MGC8392, SCHAD, L-3-hydroxyacyl-Coenzyme A dehydrogenase, L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain. Official Symbol: HADH. RefSeq number(s): NP_005318.2

Specificity: Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx. 33kDa band observed in Human Heart, Muscle and Kidney lysates (calculated MW of 34.3kDa according to NP_005318.2). In transfected HEK293 transiently expressing HADH a band of approx. 38kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03µg/ml. Full caption: HEK293 overexpressing HADH (RC201752) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with ABIN238619 in the right panel (mock transfection in first and last lanes), tested by Origene.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN238619 (0.02µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing HADH (RC201752) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel. For full caption: see Application Notes.

Publications

Publications: Molven, Matre, Duran et al.: "Familial hyperinsulinemic hypoglycemia caused by a defect in the SCHAD enzyme of mitochondrial fatty acid oxidation." in: Diabetes, Vol. 53, Issue 1, pp. 221-7, 2003 (PubMed).