alpha-Mannosidase II antibody (Internal Region)

Catalog No. ABIN238628

The Goat Polyclonal anti-alpha-Mannosidase II antibody has been validated for WB and ELISA. It is suitable to detect alpha-Mannosidase II in samples from Human.

Quick Overview for alpha-Mannosidase II antibody (Internal Region) (ABIN238628)

Target: alpha-Mannosidase II (MAN2A1) (Mannosidase, Alpha, Class 2A, Member 1 (MAN2A1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This alpha-Mannosidase II antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-alpha-Mannosidase II Antibody

Binding Specificity: Internal Region

Purpose: MAN2A1

Sequence: KISSDIKSQN R

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KISSDIKSQNR, from the internal region of the protein sequence according to NP_002363.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 150 kDa band observed in Human Placenta lysates (calculated MW of 131 kDa according to NP_002363.2). In transfected HEK293 transiently expressing MAN2A1 a band of approx. 98 kDa is observed. This band is not observed in the non-transfe

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for alpha-Mannosidase II

Target: alpha-Mannosidase II (MAN2A1) (Mannosidase, Alpha, Class 2A, Member 1 (MAN2A1))

Alternative Name: MAN2A1

Background: MAN2A1, mannosidase, alpha, class 2A, member 1, GOLIM7, MANA2, MANII, Golgi alpha-mannosidase II, golgi integral membrane protein 7, mannosidase, alpha type II, mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

Gene ID: 4124

NCBI Accession: NP_002363