Mannosidase, Alpha, Class 2A, Member 1 (MAN2A1) antibody

Antigen: Mannosidase, Alpha, Class 2A, Member 1 (MAN2A1)

Synonyms: MANA2, MANII, GOLIM7, Mana2, Manno, CG18474, CG8139, DM-GII.1, GMII, MAN-2, Man, dGMII, DmelCG18802, CG18802, MAN2A1, si:ch211-131c14.1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238628

Additional Information

Alternative name: MAN2A1

Gene ID: 4124

Immunogen: C-KISSDIKSQNR

Format: Affinity-purified

Description: All names and symbols: MAN2A1, mannosidase, alpha, class 2A, member 1, GOLIM7, MANA2, MANII, Golgi alpha-mannosidase II, golgi integral membrane protein 7, mannosidase, alpha type II, mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase. Official Symbol: MAN2A1. RefSeq number(s): NP_002363.2

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx. 150kDa band observed in Human Placenta lysates (calculated MW of 131kDa according to NP_002363.2). In transfected HEK293 transiently expressing MAN2A1 a band of approx. 98kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN238628 (0.3µg/ml) staining of Human Placenta lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing Man2A1 (RC220186) and probed with ABIN238628 (mock transfection in first lane), tested by Origene.

Publications

Publications: Green, Stone, Tenno et al.: "Mammalian N-glycan branching protects against innate immune self-recognition and inflammation in autoimmune disease pathogenesis." in: Immunity, Vol. 27, Issue 2, pp. 308-20, 2007 (PubMed).