Erythrocyte Protein Band 4.1-Like 4 (EPB41L4A) antibody

Antigen: Erythrocyte Protein Band 4.1-Like 4 (EPB41L4A)

Synonyms: NBL4, EPB41L4, FLJ38738

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238632

Additional Information

Alternative name: NBL4

Gene ID: 64097

Immunogen: C-KNENSPDTQRSKSH

Format: Affinity-purified

Description: All names and symbols: EPB41L4A, erythrocyte membrane protein band 4.1 like 4A, EPB41L4, FLJ38738, NBL4, erythrocyte protein band 4.1-like 4. Official Symbol: EPB41L4A. RefSeq number(s): NP_071423.3

Specificity: Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Preliminary experiments gave an approx 100kDa band in Human Brain (Cerebellum) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.0kDa according to NP_071423.3. The 100kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Diakowski, Grzybek, Sikorski: "Protein 4.1, a component of the erythrocyte membrane skeleton and its related homologue proteins forming the protein 4.1/FERM superfamily." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 44, Issue 4, pp. 231-48, 2007 (PubMed).