Membrane-Associated Ring Finger (C3HC4) 6, E3 Ubiquitin Protein Ligase (MARCH6) (AA 701-712) antibody

Antigen: Membrane-Associated Ring Finger (C3HC4) 6, E3 Ubiquitin Protein Ligase (MARCH6)

Binding Site: AA 701-712

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238654

Additional Information

Alternative name: TEB4 / MARCH-VI

Gene ID: 10299, 223455 (mouse)

Immunogen: C-QGRRVIFQKVKE

Description: All names and symbols: MARCH6, membrane-associated ring finger (C3HC4) 6, KIAA0597, MARCH-VI, RNF176, TEB4, membrane-associated RING-CH protein VI. Official Symbol: MARCH6. RefSeq number(s): NP_005876.2

Specificity: Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 100kDa band observed in Human Lung lysates (calculated MW of 103kDa according to NP_005876.2). Recommended concentration: 0.2-0.5μg/ml. An additional band of unknown identity was also consistently observed at 18kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN238654 (0.2 ug/ml) staining of Human Lung lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Kreft, Wang, Hochstrasser: "Membrane topology of the yeast endoplasmic reticulum-localized ubiquitin ligase Doa10 and comparison with its human ortholog TEB4 (MARCH-VI)." in: The Journal of biological chemistry, Vol. 281, Issue 8, pp. 4646-53, 2006 (PubMed).