SRY (Sex Determining Region Y)-Box 1 (SOX1) antibody

Antigen: SRY (Sex Determining Region Y)-Box 1 (SOX1)

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238657

Additional Information

Alternative name: SOX1

Gene ID: 6656

Immunogen: C-AHPHNPQPMHR

Format: Affinity-purified

Description: All names and symbols: SRY (sex determining region Y)-box 1, SRY-related HMG-box gene 1. Official Symbol: SOX1. RefSeq number(s): NP_005977.2

Specificity: Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 26kDa band in lysates of cell line KELLY after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 39.0kDa according to NP_005977.2. The 26kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Stem Cells, Transcription Factors

Restrictions: For Research Use only

Publications

Publications: Kan, Israsena, Zhang et al.: "Sox1 acts through multiple independent pathways to promote neurogenesis." in: Developmental biology, Vol. 269, Issue 2, pp. 580-94, 2004 (PubMed).