Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4) antibody

Antigen: Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4)

Clonality: Polyclonal

Host: Goat

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: ELISA

Catalog no.: ABIN238659

Additional Information

Alternative name: MARCH-IV

Gene ID: 57574

Immunogen: DFCKEKTEDRYS

Description: All names and symbols: MARCH4, membrane-associated ring finger (C3HC4) 4, MARCH-IV, MGC104908, RNF174, membrane-associated RING-CH protein IV. Official Symbol: 01.03.04. RefSeq number(s): NP_065865.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: Preliminary experiments in Human Brain (Amygdala, Cerebellum, Cerebral Cortex, Frontal Cortex) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Bartee, Mansouri, Hovey Nerenberg et al.: "Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins." in: Journal of virology, Vol. 78, Issue 3, pp. 1109-20, 2004 (PubMed).