Exonuclease 1 (EXO1) antibody

Antigen: Exonuclease 1 (EXO1)

Synonyms: ATWEX, ATWRNEXO, EXONUCLEASE, F18A5.260, F18A5_260, WERNER SYNDROME-LIKE EXONUCLEASE, WEX, WRNEXO, HEX1, hExoI, Msa, 5730442G03Rik, EXO1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238661

Additional Information

Alternative name: EXO1

Gene ID: 9156

Immunogen: C-HRNYSPRPESGT

Format: Affinity-purified

Description: All names and symbols: EXO1, exonuclease 1, HEX1, hExoI, rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1. Official Symbol: EXO1 . RefSeq number(s): NP_003677.3, NP_006018.3, NP_569082.1

Specificity: This antibody is expected to recognise all three reported isoforms (NP_003677.3, NP_006018.3 and NP_569082.1) Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 90kDa band observed in lysates in cell lines HEK293 and A549 (calculated MW of 89.1kDa according to NP_003677.3). Recommended concentration: 0.2-0.5µg/ml. An additional band of unknown identity was also consistently observed at 38kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Metabolism

Restrictions: For Research Use only

Images

ABIN238661 (0.2µg/ml) staining of A549 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Kim, Roh, Yoon et al.: "MLH3 and EXO1 alterations in familial colorectal cancer patients not fulfilling Amsterdam criteria." in: Cancer genetics and cytogenetics, Vol. 176, Issue 2, pp. 172-4, 2007 (PubMed).