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Neutral Cholesterol Ester Hydrolase 1 (NCEH1) antibody

Antigen

Neutral Cholesterol Ester Hydrolase 1 (NCEH1)

Synonyms NCEH, AADACL1, RGD1311104, Nceh, CPO-BP, Aadacl1, B230106I24Rik, NCEH1, DKFZp459K104, nceh, aadacl1, MGC79479
Clonality Polyclonal
Host
Reactivity
Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA
1 reference available
Catalog no. ABIN238671
Quantity 100 ug
Price 261.61 $   Plus shipping costs $45.00
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Additional Information

Alternative name AADACL1
Gene ID 57552, 320024 (mouse)
Immunogen C-RTRNSYIKWLDQN
Description All names and symbols: AADACL1, arylacetamide deacetylase-like 1. Official Symbol: AADACL1 . RefSeq number(s): NP_065843.3
Specificity Human, Mouse, Dog

Application Details

Application Notes Peptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Preliminary experiments gave an approx 26-28kDa band in Human Brain (Amygdala, Cerebellum, Hippocampus) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 49.1kDa according to NP_065843.3. The 26-28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions For Research Use only

Publications

Chiang, Niessen, Saghatelian et al.: "An enzyme that regulates ether lipid signaling pathways in cancer annotated by multidimensional profiling." in: Chemistry & biology, Vol. 13, Issue 10, pp. 1041-50, 2006 (PubMed).

Alternatives

Alternatives for antigen "Neutral Cholesterol Ester Hydrolase 1 (NCEH1)", type "Antibodies"
Hosts Rabbit (29), Goat (2)
Reactivities Human (29), Mouse (Murine) (25), Rat (Rattus) (21), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1)
Applications Immunofluorescence (IF) (16), Western Blotting (WB) (14), ELISA (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (IHC) (2), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes C-Term (1), Internal Region (1), N-Term,AA 85-115 (1)