Neutral Cholesterol Ester Hydrolase 1 (NCEH1) antibody

Antigen: Neutral Cholesterol Ester Hydrolase 1 (NCEH1)

Synonyms: NCEH, AADACL1, RGD1311104, Nceh, CPO-BP, Aadacl1, B230106I24Rik, NCEH1, DKFZp459K104, nceh, aadacl1, MGC79479

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN238671

Additional Information

Alternative name: AADACL1

Gene ID: 57552, 320024 (mouse)

Immunogen: C-RTRNSYIKWLDQN

Format: Affinity-purified

Description: All names and symbols: AADACL1, arylacetamide deacetylase-like 1. Official Symbol: AADACL1 . RefSeq number(s): NP_065843.3

Specificity: Human, Mouse, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Preliminary experiments gave an approx 26-28kDa band in Human Brain (Amygdala, Cerebellum, Hippocampus) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 49.1kDa according to NP_065843.3. The 26-28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Chiang, Niessen, Saghatelian et al.: "An enzyme that regulates ether lipid signaling pathways in cancer annotated by multidimensional profiling." in: Chemistry & biology, Vol. 13, Issue 10, pp. 1041-50, 2006 (PubMed).