Aldo-Keto Reductase Family 1, Member B10 (Aldose Reductase) (AKR1B10) antibody

Antigen: Aldo-Keto Reductase Family 1, Member B10 (Aldose Reductase) (AKR1B10)

Synonyms: HIS, HSI, ARL1, ARL-1, ALDRLn, AKR1B11, AKR1B12, MGC14103, 2310005E10Rik, AKR1B10, AKR, MGC179497

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238673

Additional Information

Alternative name: AKR1B10

Gene ID: 57016

Immunogen: C-QSSHLEDYPFDAE

Format: Affinity-purified

Description: All names and symbols: AKR1B10, aldo-keto reductase family 1, member B10 (aldose reductase) , AKR1B11, AKR1B12, ALDRLn, ARL-1, ARL1, HIS, HIS, MGC14103, aldo-keto reductase family 1, member B10, aldo-keto reductase family 1, member B11 (aldose reductase-like), aldose reductase-like 1N: aldose reductase-like peptide, aldose reductase-related protein, small intestine reductase. Official Symbol: AKR1B10 . RefSeq number(s): NP_064695.2

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 35kDa band observed in lysates of cell lines HEK293, A549 and HepG2 (calculated MW of 36.0kDa according to NP_064695.2). In transfected HEK293 transiently expressing AKR1B10 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer, Metabolism

Restrictions: For Research Use only

Images

ABIN238673 (0.03µg/ml) staining of A549 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing AKR1B10 (RC203177) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with ABIN238673 in the right panel (mock transfection in first and last lanes), tested by Origene.

Publications

Publications: Tammali, Ramana, Singhal et al.: "Aldose reductase regulates growth factor-induced cyclooxygenase-2 expression and prostaglandin E2 production in human colon cancer cells." in: Cancer research, Vol. 66, Issue 19, pp. 9705-13, 2006 (PubMed).