Apolipoprotein B MRNA Editing Enzyme, Catalytic Polypeptide-Like 2 (APOBEC2) (AA 12-26) antibody

Antigen: Apolipoprotein B MRNA Editing Enzyme, Catalytic Polypeptide-Like 2 (APOBEC2)

Synonyms: ARP1, ARCD1, MGC84761, arp1, arcd1, MGC89256, APOBEC2, MGC128604, DKFZp468H1727, Arp1

Binding Site: AA 12-26

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN238679

Additional Information

Alternative name: ApoBEC2

Gene ID: 10930, 11811 (mouse), 301226 (rat)

Immunogen: C-EAASQNGEDLENLDD

Description: All names and symbols: APOBEC2, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 2, ARCD1, ARP1, OTTHUMP00000039773, apolipoprotein B mRNA editing enzyme, catalytic polypeptide 2. Official Symbol: APOBEC2 . RefSeq number(s): NP_006780.1

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:1,000. Western Blot: Approx 26kDa band observed in Human Heart and Skeletal Muscle lysates (calculated MW of 25.7kDa according to NP_006780.1). Recommended concentration: 1-3µg/ml. An additional band of 90kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN238679 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Prochnow, Bransteitter, Klein et al.: "The APOBEC-2 crystal structure and functional implications for the deaminase AID." in: Nature, Vol. 445, Issue 7126, pp. 447-51, 2007 (PubMed).