NR5A2 + LRH1 antibody (AA 1-67)

Catalog No. ABIN239834

The Rabbit Polyclonal anti-NR5A2 + LRH1 antibody is suitable to detect NR5A2 + LRH1 in samples from Human. It has been validated for WB, IHC and IHC (p).

Quick Overview for NR5A2 + LRH1 antibody (AA 1-67) (ABIN239834)

Target: NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR5A2 + LRH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NR5A2 + LRH1 Antibody

Binding Specificity: AA 1-67

Specificity: Peptide corresponding to amino acids 1-67 of human CPF, member 2, variant 2

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human NR5A2.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:50), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Phosphate-buffered solution,  pH 7.2, 0.09 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Target Details for NR5A2 + LRH1

Target: NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))

Alternative Name: NR5A2 / LRH-1

Background: Name/Gene ID: NR5A2
Subfamily: NR5 Fushi tarazu-like
Family: NHR

Synonyms: NR5A2, B1F, B1F2, B1-binding factor, CPF, CYP7A promoter-binding factor, FTZ-F1beta, FTF, Liver receptor homolog 1, LRH1, LRH-1, HB1F, HB1F-2, Nuclear receptor phr-1, FTZ-F1, Liver receptor homolog-1, Nuclear receptor NR5A2, Receptor phr

Gene ID: 2494

UniProt: O00482

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway