NR5A2 + LRH1 antibody (C-Term)

Catalog No. ABIN1049146

Product Details anti-NR5A2 + LRH1 Antibody

Target: NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))

Binding Specificity: C-Term

Reactivity: Human, Cow, Sheep, Dog, Horse, Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR5A2 + LRH1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NR5A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Sheep, Bovine, Dog, Elephant, Panda, Horse, Pig (100%) Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback (94%) Mouse, Rat, Trout, Pufferfish, Zebrafish (88%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 17 amino acid peptide from C-terminal to ligand-binding domain of human NR5A2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Sheep, Bovine, Dog, Elephant, Panda, Horse, Pig (100%), Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback (94%), Mouse, Rat, Trout, Pufferfish, Zebrafish (88%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (7 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NR5A2 + LRH1

Target: NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))

Alternative Name: NR5A2 / LRH-1 (NR5A2 Products)

Synonyms: xff1rashort antibody, NR5A2 antibody, b1f antibody, b1f2 antibody, cpf antibody, ff1r antibody, ftf antibody, ftz-f1 antibody, ftz-f1beta antibody, hb1f antibody, hb1f-2 antibody, lrh-1 antibody, lrh1 antibody, xff1ra antibody, B1F antibody, B1F2 antibody, CPF antibody, FTF antibody, FTZ-F1 antibody, FTZ-F1beta antibody, LRH-1 antibody, LRH1 antibody, hB1F-2 antibody, xFF1rB antibody, AU020803 antibody, D1Ertd308e antibody, Ftf antibody, UF2-H3B antibody, Lrh-1 antibody, nuclear receptor subfamily 5 group A member 2 S homeolog antibody, nuclear receptor subfamily 5 group A member 2 antibody, nuclear receptor subfamily 5 group A member 2 L homeolog antibody, nuclear receptor subfamily 5, group A, member 2 antibody, nr5a2.S antibody, NR5A2 antibody, nr5a2 antibody, nr5a2.L antibody, Nr5a2 antibody

Background: Name/Gene ID: NR5A2
Subfamily: NR5 Fushi tarazu-like
Family: NHR

Synonyms: NR5A2, B1F, B1F2, B1-binding factor, CPF, CYP7A promoter-binding factor, FTZ-F1beta, FTF, Liver receptor homolog 1, LRH1, LRH-1, HB1F, HB1F-2, Nuclear receptor phr-1, FTZ-F1, Liver receptor homolog-1, Nuclear receptor NR5A2, Receptor phr

Gene ID: 2494

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway