NFATC1 antibody (AA 263-282)

Catalog No. ABIN241575

The Rabbit Polyclonal anti-NFATC1 antibody (ABIN241575) specifically detects NFATC1 in WB, IHC and IHC (p). The antibody is reactive with Human and Monkey samples.

Quick Overview for NFATC1 antibody (AA 263-282) (ABIN241575)

Target: NFATC1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFATC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NFATC1 Antibody

Binding Specificity: AA 263-282

Specificity: The amino acid sequence used is 90 % homologous in mouse and rat and 100 % identical in human NFATc1 A, B, C and E isoforms. The reported amino acid size of NFATc1 isoforms are as follows: A -716 aa, B-825 aa, C-930 aa, and E-812 aa.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Mouse, Rat, Rabbit, Turkey, Chicken, Lizard (89%) Marmoset, Opossum, Xenopus (84%).

Purification: Protein G purified

Immunogen: Amino acids 264-282 (NKRKYSLNGRQPPYSPHHS) of human NFATc1 were used as immunogen for this antibody. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Mouse, Rat, Rabbit, Turkey, Chicken, Lizard (89%), Marmoset, Opossum, Xenopus (84%).

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for NFATC1

Target: NFATC1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1))

Alternative Name: NFATC1

Background: Name/Gene ID: NFATC1

Synonyms: NFATC1, NFATc, NFAT2, NF-ATC, NF-ATC transcription factor, NF-ATc1

Gene ID: 4772

UniProt: O95644

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway