NKIRAS1 antibody (AA 96-113)

Catalog No. ABIN241576

This Mouse Monoclonal antibody specifically detects NKIRAS1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Dog, Goat, Horse, Rabbit, Hamster and Monkey.

Quick Overview for NKIRAS1 antibody (AA 96-113) (ABIN241576)

Target: NKIRAS1 (NFKB Inhibitor Interacting Ras-Like 1 (NKIRAS1))

Reactivity: Human, Mouse, Rat, Dog, Goat, Horse, Rabbit, Hamster, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This NKIRAS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 184C278

Product Details anti-NKIRAS1 Antibody

Binding Specificity: AA 96-113

Specificity: A synthetic peptide containing amino acid residues 96-113 of human kB-Ras1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Horse, Rabbit (100%) Bovine, Pig, Opossum, Turkey, Chicken (94%) Lizard, Xenopus (89%).

Purification: Protein G purified

Immunogen: A synthetic peptide containing amino acid residues 96-113 (RVELLKKEIDKFKDKKEV) of human kB-Ras1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Horse, Rabbit (100%), Bovine, Pig, Opossum, Turkey, Chicken (94%), Lizard, Xenopus (89%).

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for NKIRAS1

Target: NKIRAS1 (NFKB Inhibitor Interacting Ras-Like 1 (NKIRAS1))

Alternative Name: NKIRAS1

Background: Name/Gene ID: NKIRAS1
Family: Ras GTPase superfamily IPR001806

Synonyms: NKIRAS1, Kappa B-Ras protein 1, Kappa B-ras 1, KappaB-Ras1, KBRAS1

Gene ID: 28512

UniProt: Q9NYS0