PMS2 antibody (AA 623-639)

Catalog No. ABIN241578

The Mouse Monoclonal anti-PMS2 antibody (Clone 163C1251) (ABIN241578) specifically detects PMS2 in WB, IHC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for PMS2 antibody (AA 623-639) (ABIN241578)

Target: PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PMS2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 163C1251

Product Details anti-PMS2 Antibody

Binding Specificity: AA 623-639

Specificity: An approx. 85 kD band should be observed.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%).

Purification: Protein G purified

Immunogen: This antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 623-639 SSLAKRIKQLHHEAQQS of human PMS2, GenBank No. NP_000526.1. Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%).

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for PMS2

Target: PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))

Alternative Name: PMS2

Background: Name/Gene ID: PMS2

Synonyms: PMS2, HNPCC4, H_DJ0042M02.9, PMS2CL, PMSL2, PMS1 protein homolog 2

Gene ID: 5395

NCBI Accession: NP_000526

UniProt: P54278

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response