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Small Subunit X of Psii (Psbx) antibody

Antigen

Small Subunit X of Psii (Psbx)

Clonality Polyclonal
Host

Rabbit

Reactivity

Arabidopsis thaliana

Application
Western Blotting (WB)
1 reference available
Catalog no. ABIN249330
Quantity 200 ul
Price 473.20 $   Plus shipping costs $35.00
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Additional Information

Immunogen KLH-conjugated synthetic peptide dervied from Arabidopsis thaliana PsbXsequence At2g06520
Format Lyophilized
Description Plant PsbX is a nucelar encoded small 4 kDa protein associated withPhotosystem II and found in all classes of oxygenic organisms.
Specificity Predicted reactivity: di and monocots including Oryza sativa and Zea mays.

Application Details

Application Notes Recommended Dilution 1: 2500 with standard ECL on 1 µg of chlorophyll/lane (WB). Additional Information: PsbX is a small (4 kDa) and very hydrophobic subunit of PSII. Immunoblots fromSDS-gels (especially with high loading) may show higher molecular weight signalsfrom PsbX not fully detached from other subunits of PSII. The use of 15%SDS-PAGE gels with 2-4 M urea is recommended when working with This antibody.
Purity Serum
Storage store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.
Research Area Plant/Algal Physiology
Restrictions For Research Use only

Publications

Publications Garcia Cerdan, Sveshnikov, Dewez et al.: "Antisense inhibition of the PsbX protein affects PSII integrity in the higher plant Arabidopsis thaliana." in: Plant & cell physiology, Vol. 50, Issue 2, pp. 191-202, 2009 (PubMed).