XET5 Xyloglucan xyloglucosyl transferase (XTH-Xet) antibody

Antigen: XET5 Xyloglucan xyloglucosyl transferase (XTH-Xet)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Barley (Hordeum vulgare)

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN249338

Additional Information

Immunogen: two synthetic peptides from highly conserved region of Horderum vulgareXTH-Xet

Format: Lyophilized

Description: Molecular interactions between wall polysaccharides, which include cellulose anda range of non-cellulosic polysaccharides such as xyloglucans and(1,3,1,4)-β-d-glucans, are fundamental to cell wall properties. These interactionshave been assumed to be non-covalent in nature in most cases. A highly purifiedbarley xyloglucan xyloglucosyl transferase HvXET5 (EC 2.4.1.207), a member ofthe GH16 group of glycoside hydrolases, catalyses the in vitro formation ofcovalent linkages between xyloglucans and cellulosic substrates, and betweenxyloglucans and (1,3,1,4)-β-d-glucans. It is possible that XETs could link differentpolysaccharides in vivo, and hence influence cell wall strength, flexibility andporosity.

Specificity: Predicted reactivity: Triticum aestivum, Oryza sativa. Not reactive in: Zea mays.

Application Details

Application Notes: Recommended Dilution 1: 500 with standard ECL (WB) , 1: 5000 (ELISA).

Purity: Serum

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Publications

Publications: Hrmova, Farkas, Lahnstein et al.: "A Barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1,3;1,4)-beta-D-glucans." in: The Journal of biological chemistry, Vol. 282, Issue 17, pp. 12951-62, 2007 (PubMed).