XTH-Xet antibody

Catalog No. ABIN249338

The Rabbit Polyclonal anti- antibody has been validated for ELISA and WB. It is suitable to detect in samples from Barley and Oryza sativa. There is 1 publication available.

Quick Overview for XTH-Xet antibody (ABIN249338)

Target: XTH-Xet

Reactivity: Barley, Oryza sativa

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB)

Product Details

Cross-Reactivity (Details): Not reactive in: Zea mays

Predicted Reactivity: monocots

Characteristics: Expected / apparent Molecular Weight of the Antigene: 31.5 / 33 kDa

Purification: serum

Immunogen: two synthetic peptides from highly conserved region of Horderum vulgare XTH-Xet

Application Details

Application Notes: 1: 500 with standard ECL (WB) , 1: 5000 (ELISA)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References

Hrmova, Farkas, Lahnstein, Fincher: "A Barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1,3;1,4)-beta-D-glucans." in: The Journal of biological chemistry, Vol. 282, Issue 17, pp. 12951-62, (2007) (PubMed).

Target Details

Target: XTH-Xet

Background: Molecular interactions between wall polysaccharides, which include cellulose and a range of non-cellulosic polysaccharides such as xyloglucans and (1,3,1,4)-β-d-glucans, are fundamental to cell wall properties. These interactions have been assumed to be non-covalent in nature in most cases. A highly purified barley xyloglucan xyloglucosyl transferase HvXET5 (EC 2.4.1.207), a member of the GH16 group of glycoside hydrolases, catalyses the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates, and between xyloglucans and (1,3,1,4)-β-d-glucans. It is possible that XETs could link different polysaccharides in vivo, and hence influence cell wall strength, flexibility and porosity.

Molecular Weight: expected: 31.5 kDa, apparent: 33 kDa