Thylakoid Membrane Formation 1 (THF1) antibody

Antigen: Thylakoid Membrane Formation 1 (THF1)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Barley (Hordeum vulgare)

Application: Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN249346

Additional Information

Alternative name: Thylakoid membrane formation 1, THF1 (Psb29)

Immunogen: KLH-conjugated peptide derived from known sequences of Psb29 includingArabidopsis thaliana Q9SKT0

Format: Lyophilized

Description: Psb29 (THF1) is a conserved 22 kDa protein which functions in biogenesis ofphotosystem II complexes. This protein is required for organization of vesicles intomature thylakoid stacks for chloroplast development. Mediates G-proteinsignalling between the plasma membrane and the plastid.

Specificity: Predicted reactivity: dicots including:Nicotiana tabacum,Solanum tuberosum, monocots including:Oryza sativa, trees: Picea sitchensis, Populus balsamifera, moss: Physcomitrellapatens, algae: Ostreococcus sp.

Application Details

Application Notes: Recommended Dilution 1: 10 000 with standard ECL (WB), 5 µg in 300 µl (IP).

Buffer: Affinity purified serum in PBS pH 7.4

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Publications

Laemmli: "Cleavage of structural proteins during the assembly of the head of bacteriophage T4." in: Nature, Vol. 227, Issue 5259, pp. 680-5, 1970 (PubMed).

Zhang, Paakkarinen, van Wijk et al.: "Co-translational assembly of the D1 protein into photosystem II." in: The Journal of biological chemistry, Vol. 274, Issue 23, pp. 16062-7, 1999 (PubMed).