Clpb Heat Shock Protein (Hsp101) (N-Term) antibody

Antigen: Clpb Heat Shock Protein (Hsp101)

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Agave tequilana

Application: Western Blotting (WB)

Catalog no.: ABIN249354

Additional Information

Alternative name: ClpB heat shock protein, N-terminal (HSP101)

Immunogen: recombinant Hsp101 N-terminal derived from the sequence of Arabidopsisthaliana Hsp101 protein P42730

Format: Lyophilized

Description: Hsp101/ClpB is a member of HSP100 protein family. These proteins help proteinaggregates formed during heat stress to fall apart to allow them to be refolded byother chaperones. HSP101 is a cytosolic heat shock protein required foracclimation to high temperature.

Specificity: Predicted reactivity: dicots including: Glycine max, Nicotiana tabacum, Vitis vinifera, monocotsincluding: Oryza sativa.

Application Details

Application Notes: Recommended Dilution 1: 1000 with standard ECL (WB).

Purity: Serum

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

From left to right: Arabidopsis thaliana WT stressed at 38°C for 1.5 hour (1) , HSP101 null mutant (hot1-3), 2 ug of a total protein extract has been loaded per well

Publications

Publications: Banti, Mafessoni, Loreti et al.: "The heat-inducible transcription factor HsfA2 enhances anoxia tolerance in Arabidopsis." in: Plant physiology, Vol. 152, Issue 3, pp. 1471-83, 2010 (PubMed).