Phycoerythrobilin and Phycourobilin (B-Pe) antibody
Reactivity: Red algae (Porphyridium cruentum)
WB
Host: Rabbit
Polyclonal
unconjugated
(1 reference)-
- Target
- Phycoerythrobilin and Phycourobilin (B-Pe)
- Reactivity
- Red algae (Porphyridium cruentum)
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB)
- Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- Predicted Reactivity
- red algae, cyanobacteria, cryptomonads
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: 17-20 and 30-34 kDa
- Purification
- serum
- Immunogen
- native purified B-phycoerythrin of Porphyridium cruentum with attached chromophores
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- Application Notes
- 1:1000 - 5000 with standard ECL (WB)
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- For reconstitution add 200 μL of sterile water.
- Handling Advice
-
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Storage
- -20 °C
- Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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-
: "Phycobilisomes of Porphyridium cruentum: pigment analysis." in: Biochemistry, Vol. 13, Issue 14, pp. 2960-6, (1974) (PubMed).
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: "Phycobilisomes of Porphyridium cruentum: pigment analysis." in: Biochemistry, Vol. 13, Issue 14, pp. 2960-6, (1974) (PubMed).
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- Target
- Phycoerythrobilin and Phycourobilin (B-Pe)
- Background
- B-PE is a phycobiliprotein which is a building block of phycobilisomes, the light-harvesting complex, in red algae and some cyanobacteria. Phycobilisomes are attached to the stromal side of the thylakoid membrane. B-phycoerythrin has both phycoerythrobilin (on alpha and beta subunits) and phycourobilin (on the gamma subunits) chromophores.
- Molecular Weight
- 17-20 and 30-34 kDa
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