TRPV5 antibody (Internal Region)

Catalog No. ABIN263199

Cited in 1 publication. The Goat Polyclonal anti-TRPV5 antibody (ABIN263199) specifically detects TRPV5 in WB, ELISA, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for TRPV5 antibody (Internal Region) (ABIN263199)

Target: TRPV5 (Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TRPV5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Verified

Product Details anti-TRPV5 Antibody

Binding Specificity: Internal Region

Purpose: TRPV5

Sequence: SHRGWEILRQ NT

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SHRGWEILRQNT, from the internal region of the protein sequence according to NP_062815.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 85 kDa band observed in Human Pancreas lysates (calculated MW of 82.6 kDa according to NP_062815.2). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human: Kennedy

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunocytochemsitry/Immunofluorescence: This antibody has been successfully used in ICC/IF on Human: Kennedy BG et al. (2010) PMID: 20405023. This antibody has been successfully used in ICC on Mouse: Mariana Labarca et al. (2015) PMID: 2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-TRPV5 antibody (ABIN263199)

Kennedy, Torabi, Kurzawa, Echtenkamp, Mangini: "Expression of transient receptor potential vanilloid channels TRPV5 and TRPV6 in retinal pigment epithelium." in: Molecular vision, Vol. 16, pp. 665-75, (2010) (PubMed).

Target Details for TRPV5

Target: TRPV5 (Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5))

Alternative Name: TRPV5

Background: TRPV5 , transient receptor potential cation channel, subfamily V, member 5 , CAT2, ECAC1, OTRPC3, calcium transporter 2, epithelial calcium channel 1, osm-9-like TRP channel 3

Gene ID: 56302, 194352, 116469

NCBI Accession: NP_062815