G Protein-Coupled Receptor 125 (GPR125) antibody

Antigen: G Protein-Coupled Receptor 125 (GPR125)

Synonyms: AU044632, 3830613O22Rik, PGR21, TEM5L

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN263209

Additional Information

Alternative name: GPR125

Gene ID: 166647

Immunogen: CTNKSASSFKNS

Format: Affinity-purified

Description: All names and symbols: GPR125 , G protein-coupled receptor 125 , PGR21, TEM5L, TEM5-like. Official Symbol: GPR125 . RefSeq number(s): NP_660333.2

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 140kDa band observed in Human Ovary lysates (calculated MW of 146kDa according to NP_660333.2). Recommended concentration: 0.5-1.5µg/ml. An additional band of unknown identity was also consistently observed at 33kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN263209 (0.5µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.