E. Coli antibody

Catalog No. ABIN263638

This Mouse Monoclonal antibody specifically detects E. Coli in WB, EIA and Neut. It exhibits reactivity toward E. coli.

Quick Overview for E. Coli antibody (ABIN263638)

Target: E. Coli

Reactivity: E. coli

Host: Mouse

Clonality: Monoclonal

Conjugate: This E. Coli antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Neutralization (Neut)

Clone: 286-13310

Product Details anti-E. Coli Antibody

Characteristics: Synonyms: E. coli SLT-1 alpha and beta

Purification: Purified

Immunogen: SLT Toxin from E. coli O157: H7

Isotype: IgG1

Application Details

Application Notes: Toxin Neutralization: Neutralized Vero Cell toxicity of E. coli O157: H7 extracts when used ata 10 μg/mL concentration. Immunoblot: Detected SLT 1 when used at 1 μg/mL concentration. ELISA: Used as capture antibody at 20 μg/mL concentration (100 μL), Immobilizes SLT 1 fordetection by an HRP conjugate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water

Concentration: 1.0 mg/mL (prior to lyophilization)

Buffer: 10 mM Phosphate, pH 7.4 containing 0.9 % Sodium chloride.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for two weeksor (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for E. Coli

Target: E. Coli

Target Type: Bacteria

Background: Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.Synonyms: EHEC, EPEC, ETEC, Escherichia coli