Influenza A Virus antibody (FITC)

Catalog No. ABIN263819

The Mouse Monoclonal anti-Influenza A Virus antibody is suitable to detect Influenza A Virus in samples from Influenza A Virus. It has been validated for IF.

Quick Overview for Influenza A Virus antibody (FITC) (ABIN263819)

Target: Influenza A Virus

Reactivity: Influenza A Virus

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Immunofluorescence (IF)

Clone: 431

Product Details

Characteristics: Synonyms: Influenza A Virus Nucleoprotein, Influenza A virus NP

Immunogen: Purified virions

Isotype: IgG2a

Application Details

Application Notes: Suitable for use in IFA and Immunohistochemistry. A starting dilution of 1/5-1/20 is recommended. Direct FA staining of target antigen in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL (OD280nm, E0.1% = 1.3)

Buffer: 0.01 M PBS, pH 7.2 containing 10 mg/mL BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: Influenza A Virus

Target Type: Influenza Virus

Background: The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).Synonyms: Influenza A Virus, Seasonal Flu