FKBP5 antibody

Catalog No. ABIN263962

This Mouse Monoclonal antibody specifically detects FKBP5 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Rabbit and Hamster.

Quick Overview for FKBP5 antibody (ABIN263962)

Target: FKBP5 (FK506 Binding Protein 5 (FKBP5))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This FKBP5 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: Hi51B

Product Details anti-FKBP5 Antibody

Specificity: Detects an ~51 kDa protein representing FKBP51 in HeLa cell lysate. Also detects FKBP51 in whole tissue extracts from rat kidney and rat and mouse testes.

Characteristics: Synonyms: AIG6, FK506-binding protein 5, FKBP54, FF1 antigen, Rotamase, Peptidyl-prolyl cis-transisomerase FKBP5, 51 kDa FK506-binding protein, 54 kDa progesterone receptor-associatedimmunophilin, HSP90-binding immunophilin, Androgen-regulated protein 6

Purification: Epitope affinity chromatography

Immunogen: Synthetic peptide corresponding to the residues of human FKBP51

Isotype: IgG

Application Details

Application Notes: Western blot (1: 2000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for FKBP5

Target: FKBP5 (FK506 Binding Protein 5 (FKBP5))

Alternative Name: FKBP5 / FKBP51

Background: Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90 (4). Although FKBP52 and FKBP51 share ~75 % sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).Synonyms: 51 kDa FK506-binding protein, 54 kDa progesterone receptor-associated immunophilin, AIG6, Androgen-regulated protein 6, FF1 antigen, FK506-binding protein 5, FKBP54, HSP90-binding immunophilin, Peptidyl-prolyl cis-trans isomerase FKBP5, Rotamase

Gene ID: 2289

UniProt: Q13451