P110 (Mitochondrial Membrane Protein) antibody

Antigen: P110 (Mitochondrial Membrane Protein)

Clonality: Monoclonal (2G2)

Host: Mouse

Reactivity: Human

Application: Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN264326

Additional Information

Immunogen: Skeletal fracrtion of HeLa-S3 cells.

Cross-Reactivity: Human, Monkey

Isotype: IgG1  (Matching secondary antibodies)

Clone: 2G2

Description: P110 is a human mitochondrial protein with a molecular weight of 110 kDa. Closeexamination of the staining pattern in HeLa and Fanconi's Anemia cells reveal differencesin the morphology and organization of mitochondria in these two cell types. The epitopetargeted may serve as a valuable marker in the investigation of relationships betweenmitochondria and other cellular structures in human cells. The mouse monoclonal P110antibody labels mitochondria in human cells, as assessed by double staining with eitherRhodamine 123 or a polyclonal antibody to mitochondrial matrix HSP-60 proteins. The P110antigen has an approximate isoelectric point of 6. 5 that copartitions with HSP-60 proteinsduring isolation of mitochondria from HeLa cells. The P110 staining pattern in HeLa andFanconi's anaemia cells reveals differences in the morphology and organization ofmitochondria in these two cell types. In HeLa cells, mitochondria appear as individualtubular compartments of variable length and are closely associated with vimentinfilaments, particularly at the periphery of the nucleus. In Fanconi's anaemia cells,mitochondria have a filamentous shape and form an interconnected cytoplasmic reticulumrunning in parallel with both vimentin filaments and microtubules. After stabilization withaldehyde- or alcohol-based fixation protocols that optimize the preservation ofcytoskeletal components, the epitope targeted by the 2G2 antibody may serve as avaluable marker in the investigation of relationships between mitochondria and othercellular structures in human cells.

Specificity: Reacts with human and monkey mitochondria. It does not react with chicken, mouse or rat.

Application Details

Application Notes: Suitable for Western Blot (2-10 ug/ml) and Immunofluorescense. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: Lot specific

Purification: Purified

Buffer: PBS with 0.08% Sodium Azide as presevative.

Storage: Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Restrictions: For Research Use only