P110 (Mitochondrial Membrane Protein) antibody

Catalog No. ABIN187409

The Mouse Monoclonal anti-P110 (Mitochondrial Membrane Protein) antibody is suitable to detect P110 (Mitochondrial Membrane Protein) in samples from Human and Monkey. It has been validated for IF and WB.

Quick Overview for P110 (Mitochondrial Membrane Protein) antibody (ABIN187409)

Target: P110 (Mitochondrial Membrane Protein)

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB)

Clone: 2G2

Product Details

Specificity: Reacts with a mitochondrial membrane protein present in humans and monkeys. Species Reactivity: Human and monkey. Does not cross-react with mouse, rat, or chicken.

Immunogen: Skeletal fraction of HeLa-S3 cells.

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence Western blot: Use at 2-10 µg/mL. The optimal dilution for a specific application should be determined by the researcher.

Restrictions: For Research Use only

Handling

Buffer: 100 µg purified IgG at 1 mg/mL in PBS with 0.08% sodium azide as a preservative. Purified from ascites fluid and sterilized by 0.2 µM filtration.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details

Target: P110 (Mitochondrial Membrane Protein)

Background: P110 is a human mitochondrial protein with a molecular weight of 110 kDa. The epitope targeted may serve as a valuable marker in the investigation of relationships between mitochondria and other cellular structures in human cells. The mouse monoclonal P110 antibody labels mitochondria in human cells, as assessed by double staining with either Rhodamine 123 or a polyclonal antibody to mitochondrial matrix HSP-60 proteins. The P110 antigen has an approximate isoelectric point of 6.5 that co-partitions with HSP-60 proteins during isolation of mitochondria from HeLa cells. The P110 staining pattern in HeLa and Fanconi's anemia cells reveals differences in the morphology and organization of mitochondria in these two cell types. In HeLa cells, mitochondria appear as individual tubular compartments of variable length and are closely associated with vimentin filaments, particularly at the periphery of the nucleus. In Fanconi's anemia cells, mitochondria have a filamentous shape and form an interconnected cytoplasmic reticulum running in parallel with both vimentin filaments and microtubules. After stabilization with aldehyde- or alcohol-based fixation protocols that optimize the preservation of cytoskeletal components, the epitope targeted by the 2G2 antibody may serve as a valuable marker in the investigation of relationships between mitochondria and other cellular structures in human cells.