Nitrotyrosine antibody

Catalog No. ABIN264465

The Mouse Monoclonal anti-Nitrotyrosine antibody (Clone 2A12) (ABIN264465) specifically detects Nitrotyrosine in WB and IHC (fro).

Quick Overview for Nitrotyrosine antibody (ABIN264465)

Target: Nitrotyrosine

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nitrotyrosine antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2A12

Product Details anti-Nitrotyrosine Antibody

Characteristics: Synonyms: NO-Tyrosine, Nitro-Tyrosine

Purification: Purified

Immunogen: 3-Nitrotyrosine-KLH

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1/1000. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: 20 mM Sodium Phosphate, 150 mM Sodium Chloride, 50 % Glycerol, 3 mM Sodium Azide, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody can be shipped at 2-8 °C. Store (in aliquots) at -20 °C only. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Nitrotyrosine

Target: Nitrotyrosine

Target Type: Chemical

Background: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxidesignaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A (SP-A) to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis.55 kD 160 kD.Synonyms: NO-Tyrosine, Nitro-Tyrosine