Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Nitrotyrosine antibody (APC)

AA, ELISA, FACS, ICC, IF, IHC, IP, WB Host: Mouse Monoclonal 39B6 APC
Catalog No. ABIN2486191
$598.00
Plus shipping costs $45.00
100 μg
Shipping to: United States
Delivery in 3 to 4 Business Days
  • Target See all Nitrotyrosine Antibodies
    Nitrotyrosine
    Reactivity
    Please inquire
    Host
    • 31
    • 26
    • 3
    Mouse
    Clonality
    • 30
    • 29
    Monoclonal
    Conjugate
    • 30
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Nitrotyrosine antibody is conjugated to APC
    Application
    • 45
    • 27
    • 25
    • 22
    • 20
    • 15
    • 13
    • 13
    • 11
    • 11
    • 10
    • 9
    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Antibody Array (AA), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
    Specificity
    Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.
    Purification
    Protein G Purified
    Immunogen
    3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin
    Clone
    39B6
    Isotype
    IgG2a
  • Application Notes
    • WB (1:1400)
    • IHC (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    0.7 μg/ml of ABIN2486191 was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    Nitrotyrosine
    Abstract
    Nitrotyrosine Products
    Target Type
    Chemical
    Background
    Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).
You are here:
Support