5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase (ATIC) antibody

Antigen: 5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase (ATIC)

Clonality: Monoclonal (F38 P7 H9)

Host: Mouse

Reactivity: Fruit Fly (Drosophila melanogaster)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN264511

Additional Information

Characteristics: Synonyms: IMP cyclohydrolase, Inosinicase, IMP synthetase, Bifunctional purine biosynthesis proteinPURH

Alternative name: ATIC / PURH

Swiss-Prot: P31939

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with asynthetic peptide derived from the human ATIC protein and mouse myeloma Ag8563 cells. Sequence common in Frog, Fruit fly, Rat and Mouse.

Cross-Reactivity: Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG1  (Matching secondary antibodies)

Clone: F38 P7 H9

Description: The bifunctional purine biosynthesis protein PURH containsphosphoribosylaminoimidazole carboxamide formyltransferase, also designated AICARtransformylase, IMP cyclohydrolase or Inosinicase. AICAR plays an important role in purinebiosynthesis, specifically in the production of nucleotides and IMP. Defects in ATIC, thegene encoding for this protein, can cause AICArebosuria, also designated AICA-ribosiduria,an inborn error in purine biosynthesis that is neurologically cataclysmic. Individuals withAICA-rebosuria accumulate AICA-ribotide, also designated ZMP, and its derivatives inerythrocytes and fibroblasts and also excrete very large amounts of AICA-riboside in theurine. Mental retardation, epilepsy, dysmorphic features and congenital blindness are allsymptoms of this disease.

Specificity: This antibody detects Phosphoribosylaminoimidazolecarboxamide Formyltransferase(ATIC). Species: Human, Frog, Dros, Rat and Mouse.

Application Details

Application Notes: Western Blot: 1-2 µg/ml. Immunohistochemistry on Paraffin Sections (15 µg/ml). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100°C for 20min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBSwithTween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: Lot specific

Purification: Purified

Buffer: Phosphate buffered saline with 0.08% Sodium Azide as preservative.

Storage: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20°C only. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only