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Antibodies
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anti-ADAM Metallopeptidase Domain 10 (ADAM10) antibody
ADAM Metallopeptidase Domain 10 (ADAM10) antibody
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| Synonyms |
kuz, AD10, MADM, CD156c, HsT18717, kuzbanian, 1700031C13Rik, ADAM10, adam10, wu:fc03d12, ADAM-10, DKFZp459A157, LOC100219653, Adam10 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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| Catalog no. |
ABIN264988 |
| Quantity |
100 µg (0.5 mg/ml) (Variants) |
| Price |
310.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: KUZ, MADM, Kuzbanian protein homolog, Disintegrin and metalloproteinasedomain-containing protein 10, Mammalian disintegrin-metalloprotease
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Alternative name
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CD156c / ADAM10
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Swiss-Prot
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O14672
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Immunogen
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Synthetic peptide corresponding to amino acids 732-748 off the Human ADAM10. Remarks: Immungen sequence is identical in Bovine and Rat and differs from the Mouseprotein by one amino acid.
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Cross-Reactivity
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Human, Mouse (Murine), Rat (Rattus)
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Proinflammatory cytokine tumor necrosis factor-a (TNF-a) contributes to a variety ofinflammatory responses and programmed cell death. Notch receptor and its ligandparticipate in cell fate decisions during vertebrate development and are associated withseveral human disorders, including a T-cell lymphoma. TNF-a, notch and its ligand deltaare all membrane-bond molecules, which are cleaved by proteases to release matureproteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrinand metalloprotease), was recently identified to cleave TNF-a, notch and its ligand delta(1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned anddesignated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).
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Specificity
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This antibody recognizes ADAM10. Species: Human, Mouse and Rat. Others not tested.
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Application Notes
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Western Blot: 0.25-1 µg/ml. Detects an 85 kDa band can be detected in Jurkat cell lysates which may representprecursor protein. A faint 60 kDa band is also detected in some cell lines, including Jurkat,which may be processed mature protein. Positive Control/Tissue Expression: Jurkat whole cell lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0.5 mg/ml
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Purification
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Purified
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Buffer
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Phosphate buffered saline solution containing 0.02% Sodium Azide as apreservative.
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Storage
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The antibody can be shipped at ambient temperature. Store (in aliquots) at -20°C. Shelf life: one year from despatch.
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Research Area
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Proteases, Extracellular Matrix
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Restrictions
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For Research Use only
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Alternatives for antigen "ADAM Metallopeptidase Domain 10 (ADAM10)", type "Antibodies"
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Hosts
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Rabbit (18), Mouse (5), Goat (2)
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Reactivities
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Human (22), Mouse (Murine) (7), Rat (Rattus) (7), Cow (Bovine) (3)
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Applications
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Western Blotting (WB) (21), Enzyme Immunoassay (EIA) (4), Immunofluorescence (IF) (4), ELISA (3), Flow Cytometry (FACS) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), Dot Blot (Dot) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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APC (1), CF (1), PE (1)
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Epitopes
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C-Term (2)
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Alternatives 
