VSX2 antibody (C-Term)

Catalog No. ABIN265010

The Sheep Polyclonal anti-VSX2 antibody has been validated for WB and IHC (fro). It is suitable to detect VSX2 in samples from Mouse and Rat. There are 5+ publications available.

Quick Overview for VSX2 antibody (C-Term) (ABIN265010)

Target: VSX2 (Visual System Homeobox 2 (VSX2))

Reactivity: Mouse, Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This VSX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-VSX2 Antibody

Binding Specificity: AA 264-361, C-Term

Specificity: This antibody recognizes chx 10 (C-Term). Other species not tested.

Purification: Purified

Immunogen: Antibody raised against a recombinant protein corresponding to amino acids 264 to 361 derived from the C terminal of the human Chx10 protein conjugated to GST.

Isotype: IgG

Application Details

Application Notes: Western Blot: 0.5-1.0 mg/mL. Detects a 46 kDa band in mouse and rat retinal tissue lysates. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS containing 0.08 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C. Can be shipped at ambient temperature.

References for anti-VSX2 antibody (ABIN265010)

Francius, Harris, Rucchin, Hendricks, Stam, Barber, Kurek, Grosveld, Pierani, Goulding, Clotman: "Identification of multiple subsets of ventral interneurons and differential distribution along the rostrocaudal axis of the developing spinal cord." in: PLoS ONE, Vol. 8, Issue 8, pp. e70325, (2013) (PubMed).

Nittner, Lambertz, Clermont, Mestdagh, Köhler, Nielsen, Jochemsen, Speleman, Vandesompele, Dyer, Schramm, Schulte, Marine: "Synthetic lethality between Rb, p53 and Dicer or miR-17-92 in retinal progenitors suppresses retinoblastoma formation." in: Nature cell biology, Vol. 14, Issue 9, pp. 958-65, (2012) (PubMed).

Qiu, Jiang, Xiang: "A comprehensive negative regulatory program controlled by Brn3b to ensure ganglion cell specification from multipotential retinal precursors." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 13, pp. 3392-403, (2008) (PubMed).

Elshatory, Everhart, Deng, Xie, Barlow, Gan: "Islet-1 controls the differentiation of retinal bipolar and cholinergic amacrine cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 46, pp. 12707-20, (2007) (PubMed).

Osakada, Ooto, Akagi, Mandai, Akaike, Takahashi: "Wnt signaling promotes regeneration in the retina of adult mammals." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 15, pp. 4210-9, (2007) (PubMed).

Target Details for VSX2

Target: VSX2 (Visual System Homeobox 2 (VSX2))

Alternative Name: VSX2 / CHX10

Background: Chx10 is a 46 kDa homeodomain protein of the paired-like class that is essential for development of the mammalian eye. Mutations in Chx10 cause microphthalmia, a cause of congenital blindness in humans, and the ocular retardation (or) phenotype in mice. In the developing mouse retina Chx10 is expressed in retinal progenitors, while in the mature retina, Chx10 expression becomes restricted to bipolar neurons. Concurrent with these expression patterns, the Chx10-/- (or) retina is thin due to a defect in proliferation of retinal progenitors, and lacks bipolar neurons. Chx10 is also expressed in the developing brainstem, thalamus, and spinal cord.Synonyms: Ceh-10 homeodomain-containing homolog, HOX10, Homeobox protein CHX10, Visual system homeobox 2

Molecular Weight: 46 kDa

Gene ID: 12677

NCBI Accession: NP_031727

UniProt: Q61412

Pathways: Dopaminergic Neurogenesis