RIT1 antibody

Catalog No. ABIN265014

The Rabbit Polyclonal anti-RIT1 antibody (ABIN265014) specifically detects RIT1 in WB. The antibody is reactive with Human samples.

Quick Overview for RIT1 antibody (ABIN265014)

Target: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RIT1 Antibody

Specificity: This antibody reacts to RIT1.

Purification: Purified

Immunogen: His-tagged recombinant human Rit protein

Isotype: IgG

Application Details

Application Notes: Western Blot: detects recombinant Rit protein expressed in HEK-293 cells, Shows minimalcross-reactivity with Rin protein and other Ras proteins.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: The antibody can be shipped at ambient temperature. Store (in aliquots) at -20 °C only.

Target Details for RIT1

Target: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Alternative Name: RIT1

Background: Rit and its neuron-specific homologue, Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64 % amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100 % conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.Synonyms: RIBB, RIT, ROC1, Ras-like protein expressed in many tissues, Ras-like without CAAX protein 1

Gene ID: 6016

NCBI Accession: NP_008843

UniProt: Q92963

Pathways: Neurotrophin Signaling Pathway